Description
Product Overview
The Heiltropfen Methylene Blue 5% Solution is a premium analytical reagent formulated with USP‑grade methylene blue trihydrate dissolved in demineralized water. Packaged in a 100 ml dark amber glass bottle with a precision glass dropper, the solution is protected from ultraviolet light and oxidation, preserving its deep blue color and chemical stability for extended storage. Each milliliter contains 50 mg of methylene blue, and a single vertical drop (0.05 ml) delivers exactly 2.5 mg, allowing researchers to dose accurately without the need for additional weighing steps. The formulation complies with United States Pharmacopeia (USP) standards, and the product is third‑party tested for heavy‑metal contamination and microbiological purity, with certificates available on request. It is also formaldehyde free, eliminating concerns about cross‑reactivity in sensitive biological assays. The bottle dimensions (5.87 × 1.89 × 1.81 inches) and lightweight design (8.82 oz) make it convenient for both bench‑top use and field‑based applications.
![[Product front view showing all components]](https://www.cutleryco.store/wp-content/uploads/2026/07/08f8fbba38b4465fbcd72b0bd617051e.jpg)
Usage
This 5% methylene blue solution is ideal for a wide range of laboratory applications, including histological staining, viability assays, flow cytometry, and spectrophotometric analyses. Researchers typically dilute the concentrate at a ratio of 0.2 ml (four drops) per 200 ml of distilled water to obtain a working concentration of 0.5 ppm, suitable for staining cellular components or tracking metabolic activity. The solution’s high purity and lack of interfering substances make it compatible with a variety of buffers, media, and fixation protocols. Because the product is packaged in a light‑protective amber bottle, it can be stored at room temperature for months without degradation, simplifying inventory management in busy research labs. Its heavy‑metal‑free certification ensures that it will not introduce background signals in trace‑element analyses, making it a reliable choice for environmental testing, pharmaceutical quality control, and academic research.
Why Choose Us
Heiltropfen operates a GMP‑certified production facility that adheres to ISO 22000 and ISO 22716 standards, guaranteeing that every batch meets stringent quality and safety criteria. Our commitment to transparency is demonstrated by independent, ISO‑certified laboratory testing for purity, heavy‑metal content, and microbiological load. Detailed analytical reports are provided to customers upon request, reinforcing confidence in the reagent’s consistency. The amber glass bottle with a calibrated glass dropper minimizes product loss and ensures precise dosing, while the robust cardboard packaging protects the bottle during shipping. Additionally, our customer support team offers technical assistance for protocol development, troubleshooting, and regulatory compliance, ensuring that users receive comprehensive guidance throughout the product lifecycle.
Key Features
- USP grade methylene blue ensures high purity for reliable experimental results
- Amber glass bottle with glass dropper protects against light and oxidation
- Third‑party tested for heavy metals and microbiology, with certificates available
- Formaldehyde‑free formulation eliminates cross‑reactivity in sensitive assays
- Responsive technical support and detailed analytical documentation for user confidence
FAQ
What is the recommended dilution for cell staining?
For most cell‑staining protocols, mix 0.2 ml (approximately four drops) of the 5% solution with 200 ml of distilled water to achieve a 0.5 ppm working concentration. Adjust the volume proportionally for larger or smaller batches.
Is the solution compatible with common laboratory buffers?
Yes, the methylene blue solution is chemically stable in phosphate‑buffered saline (PBS), Tris‑HCl, and other standard aqueous buffers. It does not react with most metal ions, making it suitable for a variety of biochemical assays.
How long can the product be stored after opening?
When kept in its original amber glass bottle, tightly sealed, and stored at room temperature away from direct sunlight, the solution remains stable for at least six months. For extended storage, refrigeration at 2‑8 °C is recommended.
Can I obtain a certificate of analysis (CoA) for this batch?
Absolutely. Heiltropfen provides a full CoA, including heavy‑metal analysis and microbiological testing results, upon request. This documentation supports regulatory compliance and quality assurance for your laboratory.
Is the product safe for use in live‑cell assays?
Because the formulation is formaldehyde‑free and free of toxic contaminants, it is suitable for live‑cell viability assays when used at appropriate dilutions. Always follow your specific assay guidelines and perform a pilot test to confirm compatibility.
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